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Oral Cancer Screening Diagnostic Aids and Adjunctive Techniques

Heather Reneé Morse, RDH, BS; and Stefanie Vanduine, RDH, MS

September 1, 2019 Course - Expires September 30th, 2022

American Dental Hygienists' Association

Abstract

The number of cases of oral cancer being diagnosed is increasing, and dental hygienists are in a good position to offer screenings to patients. This article explains the importance of early detection and describes various screening methods, including adjunctive techniques. Tests include standard screening, established diagnostic adjuncts, light-based detection systems, and salivary biomarkers.

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Dental hygienists do not conduct oral cancer screenings as often as recommended.1 Visual and tactile screenings are the only tests to screen for oral cancer recommended by The Oral Cancer Foundation (OCF), the World Health Organization (WHO), and the National Institute of Dental and Craniofacial Research (NIDCR).2,3 When abnormal lesions are detected, it is recommended they be reevaluated after 2 weeks and then considered for biopsy.3-5 Dental hygienists also need to be aware of the oral cancer screening diagnostic aids and adjunctive techniques that are available; however, these methods are purely an adjunct to oral cancer screenings. This review of the literature discusses the incidence of oral cancer, the significance of early detection, and the types of diagnostic aids and adjunctive techniques available to screen for oral cancer.

Oral cancer is a serious health issue that is globally on the rise.6 The WHO estimates 657,000 new cases of cancer of the oral cavity and pharynx will be diagnosed worldwide each year, and more than 330,000 deaths will occur.7 In the United States, an estimated 53,000 Americans will be diagnosed with oral or oropharyngeal cancer in 2019.6 Oral cancer is twice as likely to occur in male patients as in female patients and usually occurs after the age of 40.6,8 Squamous cell carcinomas account for 95 percent of the malignancies of the oral cavity, with the remainder being adenocarcinomas, adenoid cystic tumors, lymphomas, and melanomas.9

Risk factors include tobacco and alcohol use, exposure to sunlight and x-rays, human papilloma virus (HPV) infections, and poor nutrition.6 Early detection of oral cancer is crucial for patient survival. If not diagnosed in its earliest stages, most oral cancers have a 5-year survival rate of less than 33 percent for blacks versus 55 percent for whites.6 Early detection of oral cancer is the most significant way to improve these survival rates.10 Rates of oral cancer in young adults are on the rise, partially linked to smokeless tobacco use, drug use, and HPV, particularly HPV16.6

Dental hygienists are educated to conduct oral cancer screenings on every patient; however, research has found that less than half of surveyed dental hygienists reported always conducting oral cancer screenings.1 In addition, there are significant knowledge gaps among dental hygienists regarding oral cancer risk factors.

Significance of Early Detection

Detecting oral cancer in its earliest stages increases the 5-year survival rate from less than 30 percent to over 80 percent.11,12 Healthy People 2020 is calling for a 10 to 15 percent reduction in death rates from oral cancer by the year 2020.13 Dental hygienists can play a significant role in detecting early signs of oral cancer and helping to reduce oral cancer death rates; however, they need to be properly educated and trained in screening processes. Delayed detection of oral cancer by dental hygienists can result from the lack of standardized oral cancer examinations, lack of knowledge, and the difficulty of visualizing abnormal tissues in the mouth.14

A variety of diagnostic aids and adjunctive techniques can be used to screen for oral cancer. These tests include the standard screening test, the oral cytology test, vital tissue staining, light-based detection systems and the salivary biomarker test. Each test uses its own unique method to visually screen and/or detect abnormalities in the oral cavity.

Types of Diagnostic Aids and Adjunctive Techniques

Table I compares the modes, sensitivities and specificities of the types of diagnostic aids and adjunctive techniques described below.

Standard Screening Test

The conventional oral examination is the only recommended test to screen for oral cancer by the OCF, the WHO, and the NIDCR.2,3 The conventional oral examination includes visually inspecting the face, neck, lips, labial mucosa, buccal mucosa, gingiva, floor of the mouth, tongue, and palate, and palpating the regional lymph nodes, tongue, and floor of the mouth.3 Abnormal lesions should be reevaluated after 2 weeks and considered for biopsy.3-5Conventional oral examinations are effective screening methods for melanoma, with sensitivity and specificity as high as 98 percent.15-17 However, they are limited in their ability to detect pre-cancerous or other malignant lesions.15,18-20 Furthermore, they are limited in their ability to detect HPV-related oropharyngeal cancers, since these cancers often originate deep within tissues where they cannot be seen with or without a light.2 A survey found only 66 percent of surveyed dental hygienists conducted conventional oral examinations and only 50 percent performed bimanual neck palpation in their screenings.11,12,21

Established Diagnostic Adjuncts

The oral cytology test, commercially marketed as the OralCDx brush biopsy (BrushTest, CDx, Suffern, New York), uses a brush to collect tissue samples from inside the mouth.22 Samples are sent to a lab and read by a cytopathologist.22 Samples are reported as "negative or benign," "positive," or "atypical."22 When "positive" or "atypical" results are determined, a scalpel biopsy is recommended.22

A study found OralCDx had 100 percent sensitivity and 100 percent specificity with Class I and Class II lesions when positive test results were considered indicative of cancer (see Glossary at the end of this article). This study also reported 92.9 percent specificity with "atypical" or "positive" test results when considered indicative of cancer.15 The study compared 80 patients who had both a brush cytology and a scalpel biopsy and found the brush technique had a sensitivity of 92 percent and a specificity of 94 percent for both "positive" and "atypical" results in detecting dysplasia and oral cancer.15 Research has found the OralCDx brush has a higher sensitivity and specificity in comparison with conventional oral examinations; however, a major limitation of oral cytology tests is that they can detect only abnormal lesions that can be seen by a dental hygienist.23

Vital tissue staining, commercially marketed as toluidine blue staining, methylene blue staining, Rose Bengal staining and Lugol's iodine staining, each use their own dye to stain abnormal tissues in the mouth.22 Abnormal tissues could potentially signify premalignant and malignant lesions, potentially malignant disorders, or both.22,24 Similar to the oral cytology tests, when abnormal tissues are detected, a scalpel biopsy is recommended.22

A study found the sensitivity of toluidine blue staining in detecting high-risk lesions was 94 percent and the specificity was 45 percent. For carcinoma, sensitivity was 100 percent and specificity was 39 percent.25 A systematic review looked at 15 studies that used toluidine blue staining.

Of the 15 studies, there were six studies in which toluidine blue staining was assessed as an adjunctive technique in lower-risk populations. There was one study using toluidine blue staining to survey for mucosal changes in a population with prior treatment for upper aerodigestive cancer with no a priori knowledge of lesions. Investigators in eight studies assessed toluidine blue staining as a diagnostic adjunct in subjects with suspicious lesions or histologically proven dysplasia or cancer in patients at higher risk.22,26-40

This study found the sensitivities varied from 38 to 98 percent, specificities varied from 9 to 93 percent, positive predictive values (PPVs) ranged from 33 to 93 percent, and negative predictive values ranged from 22 to 92 percent.22 According to Messadi, "[The toluidine blue staining] test appears to be highly sensitive (93.5 percent to 97.8 percent) but less specific (73.3 percent to 92.9 percent), mainly because of high false-positive results."10,22,41 Toluidine blue staining does have limitations, including selective staining, which causes some premalignant and malignant lesions to go undetected.25 Furthermore, it has a low specificity rate because it generates false positives.23 As a result of these limitations, a conventional oral examination is recommended prior to all vital tissue staining tests.23

Light-based Detection Systems

ViziLite (Denmat Holdings LLC, Lompoc, California) uses a blue/white light to visualize abnormal cells that have been prepared with acetic acid.22 The patient rinses with acetic acid and the clinician places the ViziLite in the patient's mouth to look for an "acetowhite" change to the tissues.22 An acetowhite change could suggest premalignant and malignant lesions.22 When acetowhite changes are detected, a scalpel biopsy is recommended.22

Two studies using ViziLite found it enhanced the visual parameters of oral lesions in "brightness, sharpness (margin delineation), surface texture and, in some cases, size of lesion compared with results of examination by means of standard illumination" as compared with conventional oral examination.22 In a study using 40 patients with a previous history of oral cancer or premalignancy, ViziLite resulted in a sensitivity of 100 percent and a specificity of 14 percent.15,40 In another study, 501 patients over the age of 40 with a positive history of tobacco use were examined by conventional oral examination. Among the 127 "suspicious" lesions detected, 77 (61 percent) were enhanced by ViziLite examination, while only 21 (5.8 percent) of the 363 "non-suspicious" lesions were ViziLite positive.15,42

The MicroLux DL system (AdDent, Inc., Danbury, Connecticut) uses a "blue-white light-emitting diode (LED)" to detect abnormal tissues.22 The patient rinses with acetic acid, then a clinician places the MicroLux DL system in the patient's mouth to look for any changes in the tissues.22 Tissue changes could suggest premalignant and malignant lesions.22 If tissue changes are found, a scalpel biopsy is recommended.22

A study using 50 patients found that lesion visibility during conventional oral examination ranked an average of 3.28, increasing to 3.66 during Microlux DL examination. This study also determined that the Microlux DL system increased border distinctness from 26 to 35 of the cases.43 Ibrahim et al studied 599 tobacco users and found the sensitivity of the MicroLux DL system to be 94.3 percent and the specificity to be 99.6 percent. This study also found that the MicroLux DL system enhanced detection of lesions, as well as discovered new lesions, as compared with conventional oral examination.44

The VELscope system (LED Dental, Atlanta, Georgia) uses a blue light to look for changes in tissue fluorescence.22 The clinician peers through the device's lens to look for a loss of fluorescence in tissues.22 If a lack of fluorescence is detected, a scalpel biopsy is recommended.22 A total of 44 patients with a history of oral dysplasia or head and neck squamous cell carcinoma were screened by conventional oral examination and then by the VELscope system, which demonstrated a "98 percent sensitivity and a 100 percent specificity for discriminating dysplasia cancers from normal oral mucosa."15,45

A survey was conducted using 30 participants who were either cigarette smokers or cigarette smokers who consumed alcohol. This survey found that there were no statistically significant differences in detecting potentially malignant lesions between using VELscope when compared with conventional oral examinations.14 Research has determined that VELscope has a higher sensitivity (100 percent versus 17 percent) but a lower specificity (74 percent versus 97 percent) when compared with conventional oral examinations.23 Mehrotra et al determined ViziLite and VELscope, in combination with conventional oral examination, were not effective in identifying dysplasia or cancer.46 These false negatives allow for large numbers of premalignant and malignant lesions to go undetected.46

Photodynamic diagnosis uses fluorescence to detect tissue changes in potential premalignant and malignant lesions.24,47 An acid rinse induces fluorescence in tissues in the mouth.24,48 If fluorescence is detected, a scalpel biopsy is recommended.24,49

A meta-analysis of 11 studies found the estimated sensitivity of photodynamic diagnosis was 91 percent and the specificity was 58 percent.24,50 A study looked at 16 patients with neoplastic lesions and determined that fluorescence was detected in the oral mucosa of all patients after the use of an acid rinse.51 Several studies have determined that photodynamic diagnosis has high sensitivity, but limited specificity, as well as a high false-positive rate.24,48,52,53

Light-based detection systems are unable to detect HPV-related oropharyngeal cancers due to the fact "these cancers start deep within the tissues beyond a level that these lights can reach."2

Salivary Biomarkers

Salivary biomarker tests are used to diagnose and predict risk factors for oral potentially malignant disorders.24 Saliva is collected from patients using an oral swab and then sent to a lab for "genomic profiling."54 Genomic profiling includes testing for oral cancer salivary biomarkers including, but not limited to, growth factors, cytokines, and epithelial tumor factors.54 Salivary biomarker testing allows for a noninvasive and cost-effective approach to the diagnosis of oral cancer.46 According to Mehrotra et al, "the six most studied epithelial serum circulatory tumor markers in the saliva of carcinoma patients are Cyfra 21-1, TPS, carcinoembryonic antigen (CEA), SCC, CA125, and CA19-9."46 The presence of Cyfra 21-1, TPS, and CA125 in salivary concentrations was significantly increased and had a sensitivity of 71 percent, a specificity of 75 percent, a negative predictive value of 71 percent, and a positive predictive value of 75 percent.46 Several studies have used salivary proteins as potential diagnostic markers for oral cancer. One study found higher levels of saliva-soluble CD44 in the majority of patients with OSCC.55,56 These higher levels could help differentiate cancer from health with high specificity.55,56

Conclusion

With nearly 657,000 new cases of cancer of the oral cavity and pharynx being diagnosed each year worldwide, and with oral cancer in young adults on the rise, oral cancer is a serious and growing problem.6,7 With early detection, the 5-year survival rate for oral cancer drastically increases from less than 30 percent to over 80 percent.11,12 There are limitations with all the various oral cancer detecting systems available on the market today. While conventional oral examinations have a high sensitivity and specificity, they are limited in their ability to detect premalignant and malignant lesions.15-20 Oral cytology biopsies and vital tissue staining have high sensitivity and specificity, but each has its own major limitations.10,15,22,23,25,41 ViziLite, MicroLux DL, VELscope, and photodynamic diagnosis all show some success in detecting oral cancer lesions; however, a scalpel biopsy is still recommended when abnormal results are detected.22 Salivary biomarker testing is a new technology that shows potential in diagnosing oral cancer.

While each test shows potential in screening for oral cancer, evidence does not support one test over another. Further research is needed to determine a single test for the detection of oral cancer. Until then, it is the professional responsibility of every dental hygienist to conduct thorough conventional oral examinations on every patient and to understand utility and limitations of diagnostic aids and adjunctive techniques.

Glossary

Acetowhite - The areas that stain white after an acetic acid wash is applied are said to have "acetowhite" changes.

Fluorescence - Mucosal tissues have a reflective and absorptive pattern based on naturally occurring fluorophores in the tissue. Exposure to blue light spectra may maximize a differential profile in area undergoing neoplastic change in which a loss of fluorescence visualization is reported.

Sensitivity - The probability that someone who has the target disease (an oral premalignant or malignant lesion [OPML]) will generate a positive result (an OPML as demonstrated by means of gold-standard tissue biopsy).

Specificity - The probability that someone who does not have an OPML will generate a negative test finding.

About the Authors

Heather R. Morse, RDH, BS, is a practicing dental hygienist in Battle Creek, Michigan. She is currently completing her Master of Science in Dental Hygiene degree at the University of Michigan.

Stefanie VanDuine, RDH, MS, graduated from the University of Michigan with a Bachelor of Science in Dental Hygiene degree in 2010 and a Master of Science in Dental Hygiene degree in 2014. She is currently teaching in both the clinical and didactic settings at the University of Michigan.

References

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Table I. Types of Diagnostic Aids and Adjunctive Techniques

Table I

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SOURCE: American Dental Hygienists' Association | September 2019

Learning Objectives:

  • Explain the importance of early detection of oral cancer.
  • Describe the types of diagnostic aids and adjunctive techniques available to screen for oral cancer.
  • Discuss the effectiveness of methods of screening for early detection of oral cancer.

Disclosures:

The author reports no conflicts of interest associated with this work.

Queries for the author may be directed to jromano@aegiscomm.com.